Immobilization of Pepsin onto Chitosan Silica Nanobeads with Glutaraldehyde as Crosslink Agent
نویسندگان
چکیده
منابع مشابه
Antifouling activity of enzyme‐functionalized silica nanobeads
The amelioration of biofouling in industrial processing equipment is critical for performance and reliability. While conventional biocides are effective in biofouling control, they are potentially hazardous to the environment and in some cases corrosive to materials. Enzymatic approaches have been shown to be effective and can overcome the disadvantages of traditional biocides, however they are...
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The objective of this work was to study enzyme immobilization on chitosan activated with glutaraldehyde, aiming to produce a cheap biocatalyst. Two different immobilization strategies were studied: one-point and multipoint covalent attachment to the solid matrix. The multipoint covalent attachment derivative had an 82% immobilization yield. It was 4.9-fold more stable than the free enzyme at 50...
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Keratinases are exciting keratin-degrading enzymes; however, there have been relatively few studies on their immobilization. A keratinolytic protease from Chryseobacterium sp. kr6 was purified and its partial sequence determined using mass spectrometry. No significant homology to other microbial peptides in the NCBI database was observed. Certain parameters for immobilization of the purified ke...
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hLF1-11 (GRRRRSVQWCA) is an antimicrobial peptide (AMP) with high activity against methicillin-resistant Staphylococcus aureus (MRSA), the most prevalent species in implant-associated infection. In this work, the effect of the surface immobilization on hLF1-11 antimicrobial activity was studied. Immobilization was performed onto chitosan thin films as a model for an implant coating due to its r...
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low stability, high cost and high selectivity towards the reaction conditions, they are limited to industrial applications [1-3]. In order to facilitate their usage in industrial applications several developments have been done in the field of enzyme technology during the last two decades [4]. Immobilization of enzymes on solid supports has become most familiar technique after Nelson and Griffi...
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ژورنال
عنوان ژورنال: Bulletin of Chemical Reaction Engineering & Catalysis
سال: 2014
ISSN: 1978-2993
DOI: 10.9767/bcrec.9.3.7060.263-269